Coding

Part:BBa_K3450004:Design

Designed by: Priya Sharma Shashaank G. Prerita Chawla   Group: iGEM20_IISER_Bhopal   (2020-10-23)


Pdx1: A transcription factor required for embryonic development of pancreas


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 705
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 258
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Escherichia coli.

Removed the SapI site from the sequence to make it RFC1000 compatible.

Source

Human genome

References